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plv teto tfap2c addgene  (Addgene inc)


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    Addgene inc plv teto tfap2c addgene
    Plv Teto Tfap2c Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 14770 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plv+teto+tfap2c+addgene/pMD2%2EG+(Plasmid+%2312259)/pm41233591-385-117-118
    Average 98 stars, based on 14770 article reviews
    plv teto tfap2c addgene - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Recombinant:

    Article Title: A primordial germ cell-like-cell platform enables CRISPRi screen for epigenetic fertility modifiers.
    Article Snippet: Reagent/resource Reference or source Identifier or Cat# Snrpn F Bisulfite sequencing TATGTAATATGATATAGTTTAGAAATTAG Snrpn R Bisulfite sequencing AATAAACCCAAATCTAAAATATTTTAATC Snrpn nest F Bisulfite sequencing AATTTGTGTGATGTTTGTAATTATTTGG Snrpn nest R Bisulfite sequencing ATAAAATACACTTTCACTACTAAAATCC H19 F Bisulfite sequencing GAGTATTTAGGAGGTATAAGAATT H19 R Bisulfite sequencing ATCAAAAACTAACATAAACCCCT H19 nest F Bisulfite sequencing GTAAGGAGATTATGTTTATTTTTGG H19 nest R Bisulfite sequencing CCTCATTAATCCCATAACTAT Dnmt3a qF qPCR GACTCGCGTGCAATAACCTTAG Dnmt3a qR qPCR GGTCACTTTCCCTCACTCTGG Dnmt3b qF qPCR CTCGCAAGGTGTGGGCTTTTGTAAC Dnmt3b qR qPCR CTGGGCATCTGTCATCTTTGCACC Ncor2 qF qPCR GATGACCCCATGAAGGTCTACA Ncor2 qR qPCR GGCCAAAGTTCTTAGGGTGCT Gapdh qF qPCR CTTTGTCAAGCTCATTTCCTGG Gapdh qR qPCR TCTTGCTCAGTGTCCTTGC Miseq_Adaptor F MiSeq AATGATACGGCGACCACCGAG ATCTACACACACTCTTTCCCTA CACGACGCTCTTCCGATCTTAT CTTGTGGAAAGGACGAAACACCG Miseq_Adaptor R MiSeq CAAGCAGAAGACGGCATACGAGAT GTGACTGGAGTTCAGACGTGTGCT CTTCCGATCTCTTTAGTTTGTATGT CTGTTGCTATTATGTCTACTATTCTTTCC seqRead Miseq MiSeq GACGCTCTTCCGATCTTATCTTGTGGAA AGGACGAAACACCG CRISPRi F CRISPRi cloning AGGCACTTGCTCGTACGACG CRISPRi R CRISPRi cloning TTAAGGTGCCGGGCCCACAT dCAS9 F dCas9-KRAB expressing CAAGGTGCTGGGCAACACCGA dCAS9 R dCas9-KRAB expressing TCGTCCACCTTGGCCATCTCG Nanog RT F Reverse transcription PCR ACCTGAGCTATAAGCAGGTTAAGAC Prdm1 RT F Reverse transcription PCR gtgacctccatggtggagaag Prdm14 RT F Reverse transcription PCR ggagagaagcccttcaagtgc bGH pA R Reverse transcription PCR GGGGGAGGGGCAAACAACA Tfap2c RT F Reverse transcription PCR GGGGACGGAATGAGATGGC WPRE R Reverse transcription PCR AAGGCATTAAAGCAGCGTATCC rtTAM2 F Reverse transcription PCR ATGTCTAGACTGGACAAGAGC rtTAM2 R Reverse transcription PCR CAGAAAGTCTTGCCATGAC Antibodies DDX4 (Rb) Abcam ab13840 DDX4 (Ms) Abcam ab27591 PLZF (Ms) Active motif 39987 GCNA (Rat) Abcam ab82527 DAZL (Rb) ThermoFisher PA5-116570 NANOS3 (Rb) Abcam ab70001 STRA8 (Rb) Abcam ab49602 SOX9 (Rb) Millipore AB5535 ZO1 (Rb) ThermoFisher 40-2200 18 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 17, 2025 from IP 2001:4490:4ead:df7f:1fb:229c:1089:e555. .. Reagent/resource Reference or source Identifier or Cat# E-Cadherin (Ms) TaKaRa M108 SSEA1 (Ms) Millipore MAB4301 SOX2 (Ms) ThermoFisher MA1-014 H3K9me3 (Rb) Upstate 07-442 H3K27me3 (Rb) EPIGENTEK A-4039-025 Goat anti-Mouse-488 Invitrogen A11001 Goat anti-Rabbit-594 Invitrogen A11012 Goat anti-Rat-594 Invitrogen A11007 Beta-actin (Ms) Sigma 1978 CAS9 (Ms) Abcam ab191468 H3K27ac (Rb) Abcam ab4729 H3 (Rb) Abcam ab1791 Goat anti-Mouse IgG, HRP ThermoFisher 31430 Anti-rabbit IgG, HRP Cell signaling 7074S PE (Phycoerythrin) mouse/rat anti-CD61 Invitrogen 12-0611-82 Alexa Fluor® 647 mouse/human anti-SSEA1 Biolegend 125608 Recombinant DNA pPBhCMV1-Prdm1-pA This study N/A pPBhCMV1-Prdm14-pA This study N/A pPBhCMV1-Nanog-pA Prof. M. Azim Surani N/A PCR4-Shh::lacZ-H11 Addgene #139098 pPyCAG-Pbase Prof. M. Azim Surani N/A pPB-CAG-rtTA-IN Addgene #60612 pSpCas9(BB)-2A-PuroR Addgene #62988 psPAX2 Addgene #12260 pMD2.G Addgene #12259 pLV-tetO-Tfap2c Addgene #70269 pLX_311-KRAB-dCas9 Addgene #96918 pGEM-T Easy vector Promega A1360 pXPR_050 Addgene #96925 Chemicals and other reagents TransIT®-LT1 Transfection Reagent Mirus BioTM MIR2304 GeneticinTM Life Technologies 10131035 Zeocin® InvivoGen ant-zn-05 LIF Peprotech 300-05 PD0325901 Reprocell 04-0006-02 CHIR99021 Reprocell 04-0004-02 poly-L-ornithine Sigma-Aldrich P3655 laminin Corning 354232 bovine plasma fibronectin Sigma-Aldrich F1141 Activin A Peprotech 120-14E bFGF Gibco 13256-029 KSR ThermoFisher 10828010 Dox Sigma D9891 Propidium iodide Invitrogen P3566 © The Author(s) EMBO reports 19 D ow nloaded from https://w w w .em bopress.org on N ovem ber 17, 2025 from IP 2001:4490:4ead:df7f:1fb:229c:1089:e555. .. Reagent/resource Reference or source Identifier or Cat# Goat serum Sigma NS02L GDNF PeproTech 450-44 Insulin-Transferrin-Selenium ThermoFisher Scientific 41400045 MEMα ThermoFisher Scientific 12571063 DeadEndTM Fluorometric TUNEL System Promega G3250 Vector Red Alkaline Phosphatase Substrate Kit Vector Laboratories SK-5100 β-Galactosidase Staining Kit Cell Signaling Technology #9860 Vectashield antifade mounting medium Vector H-1000 EZ DNA methylation-lightning kits ZYMO Research D5030 E.Z.N.A.® Total RNA Kit I Omega BIO-TEK R6834 TRIzol® reagent ThermoFisher Scientific 15596018 qScriptTM cDNA SuperMix kit Quantabio 95048 RT2 SYBR® Green qPCR Mastermixes Qiagen 33050 T7 ligase NEB M0318S Bsmb1 NEB R0580 Stbl4 competent cells Invitrogen 11635018 Blasticidin Gibco A1113903 Puromycin Gibco A1113803 Genomic DNA Purification Kit Thermo Scientific K0722 Q5 High-Fidelity DNA Polymerase NEB M0491L Gel Extraction Kit Qiagen 28704 control siNT Dharmacon D-001810-10-05 siNcor2 Dharmacon L-045364-00-0005 jetPRIME transfection reagent Polyplus 101000046 Sodium butyrate Sigma B5887 valproic acid Sigma P4543 protease inhibitors Sigma 04693159001 RIPA buffer ThermoFisher Scientific 89900 Phosphatase inhibitors Sigma 4906845001 BCA Protein Assay Kit Pierce 23250 Gradient polyacrylamide gel Bio-Rad 4561083EDU PVDF membrane Millipore IPVH00010 HRR substrate Millipore WBLUC0500 NEBNext Ultra II Directional RNA Library Prep Kit NEB E7765 Software Epifactors https://epifactors.autosome.org/ GPP sgRNA Design tool https://portals.broadinstitute.org/gpp/public/analysis-tools/ sgrna-design ScreenProcessing Tools https://github.com/mhorlbeck/ScreenProcessing MAGeCK, MAGeCK-VISPR, and MAGeCKFlute Tools https://github.com/WubingZhang/MAGeCKFlute DESeq2 https://bioconductor.org/packages/release/bioc/html/ DESeq2.html SARTools https://github.com/PF2-pasteur-fr/SARTools RRHO2 https://github.com/RRHO2/RRHO2 20 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 17, 2025 from IP 2001:4490:4ead:df7f:1fb:229c:1089:e555.

    Transfection:

    Article Title: A primordial germ cell-like-cell platform enables CRISPRi screen for epigenetic fertility modifiers.
    Article Snippet: Reagent/resource Reference or source Identifier or Cat# Snrpn F Bisulfite sequencing TATGTAATATGATATAGTTTAGAAATTAG Snrpn R Bisulfite sequencing AATAAACCCAAATCTAAAATATTTTAATC Snrpn nest F Bisulfite sequencing AATTTGTGTGATGTTTGTAATTATTTGG Snrpn nest R Bisulfite sequencing ATAAAATACACTTTCACTACTAAAATCC H19 F Bisulfite sequencing GAGTATTTAGGAGGTATAAGAATT H19 R Bisulfite sequencing ATCAAAAACTAACATAAACCCCT H19 nest F Bisulfite sequencing GTAAGGAGATTATGTTTATTTTTGG H19 nest R Bisulfite sequencing CCTCATTAATCCCATAACTAT Dnmt3a qF qPCR GACTCGCGTGCAATAACCTTAG Dnmt3a qR qPCR GGTCACTTTCCCTCACTCTGG Dnmt3b qF qPCR CTCGCAAGGTGTGGGCTTTTGTAAC Dnmt3b qR qPCR CTGGGCATCTGTCATCTTTGCACC Ncor2 qF qPCR GATGACCCCATGAAGGTCTACA Ncor2 qR qPCR GGCCAAAGTTCTTAGGGTGCT Gapdh qF qPCR CTTTGTCAAGCTCATTTCCTGG Gapdh qR qPCR TCTTGCTCAGTGTCCTTGC Miseq_Adaptor F MiSeq AATGATACGGCGACCACCGAG ATCTACACACACTCTTTCCCTA CACGACGCTCTTCCGATCTTAT CTTGTGGAAAGGACGAAACACCG Miseq_Adaptor R MiSeq CAAGCAGAAGACGGCATACGAGAT GTGACTGGAGTTCAGACGTGTGCT CTTCCGATCTCTTTAGTTTGTATGT CTGTTGCTATTATGTCTACTATTCTTTCC seqRead Miseq MiSeq GACGCTCTTCCGATCTTATCTTGTGGAA AGGACGAAACACCG CRISPRi F CRISPRi cloning AGGCACTTGCTCGTACGACG CRISPRi R CRISPRi cloning TTAAGGTGCCGGGCCCACAT dCAS9 F dCas9-KRAB expressing CAAGGTGCTGGGCAACACCGA dCAS9 R dCas9-KRAB expressing TCGTCCACCTTGGCCATCTCG Nanog RT F Reverse transcription PCR ACCTGAGCTATAAGCAGGTTAAGAC Prdm1 RT F Reverse transcription PCR gtgacctccatggtggagaag Prdm14 RT F Reverse transcription PCR ggagagaagcccttcaagtgc bGH pA R Reverse transcription PCR GGGGGAGGGGCAAACAACA Tfap2c RT F Reverse transcription PCR GGGGACGGAATGAGATGGC WPRE R Reverse transcription PCR AAGGCATTAAAGCAGCGTATCC rtTAM2 F Reverse transcription PCR ATGTCTAGACTGGACAAGAGC rtTAM2 R Reverse transcription PCR CAGAAAGTCTTGCCATGAC Antibodies DDX4 (Rb) Abcam ab13840 DDX4 (Ms) Abcam ab27591 PLZF (Ms) Active motif 39987 GCNA (Rat) Abcam ab82527 DAZL (Rb) ThermoFisher PA5-116570 NANOS3 (Rb) Abcam ab70001 STRA8 (Rb) Abcam ab49602 SOX9 (Rb) Millipore AB5535 ZO1 (Rb) ThermoFisher 40-2200 18 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 17, 2025 from IP 2001:4490:4ead:df7f:1fb:229c:1089:e555. .. Reagent/resource Reference or source Identifier or Cat# E-Cadherin (Ms) TaKaRa M108 SSEA1 (Ms) Millipore MAB4301 SOX2 (Ms) ThermoFisher MA1-014 H3K9me3 (Rb) Upstate 07-442 H3K27me3 (Rb) EPIGENTEK A-4039-025 Goat anti-Mouse-488 Invitrogen A11001 Goat anti-Rabbit-594 Invitrogen A11012 Goat anti-Rat-594 Invitrogen A11007 Beta-actin (Ms) Sigma 1978 CAS9 (Ms) Abcam ab191468 H3K27ac (Rb) Abcam ab4729 H3 (Rb) Abcam ab1791 Goat anti-Mouse IgG, HRP ThermoFisher 31430 Anti-rabbit IgG, HRP Cell signaling 7074S PE (Phycoerythrin) mouse/rat anti-CD61 Invitrogen 12-0611-82 Alexa Fluor® 647 mouse/human anti-SSEA1 Biolegend 125608 Recombinant DNA pPBhCMV1-Prdm1-pA This study N/A pPBhCMV1-Prdm14-pA This study N/A pPBhCMV1-Nanog-pA Prof. M. Azim Surani N/A PCR4-Shh::lacZ-H11 Addgene #139098 pPyCAG-Pbase Prof. M. Azim Surani N/A pPB-CAG-rtTA-IN Addgene #60612 pSpCas9(BB)-2A-PuroR Addgene #62988 psPAX2 Addgene #12260 pMD2.G Addgene #12259 pLV-tetO-Tfap2c Addgene #70269 pLX_311-KRAB-dCas9 Addgene #96918 pGEM-T Easy vector Promega A1360 pXPR_050 Addgene #96925 Chemicals and other reagents TransIT®-LT1 Transfection Reagent Mirus BioTM MIR2304 GeneticinTM Life Technologies 10131035 Zeocin® InvivoGen ant-zn-05 LIF Peprotech 300-05 PD0325901 Reprocell 04-0006-02 CHIR99021 Reprocell 04-0004-02 poly-L-ornithine Sigma-Aldrich P3655 laminin Corning 354232 bovine plasma fibronectin Sigma-Aldrich F1141 Activin A Peprotech 120-14E bFGF Gibco 13256-029 KSR ThermoFisher 10828010 Dox Sigma D9891 Propidium iodide Invitrogen P3566 © The Author(s) EMBO reports 19 D ow nloaded from https://w w w .em bopress.org on N ovem ber 17, 2025 from IP 2001:4490:4ead:df7f:1fb:229c:1089:e555. .. Reagent/resource Reference or source Identifier or Cat# Goat serum Sigma NS02L GDNF PeproTech 450-44 Insulin-Transferrin-Selenium ThermoFisher Scientific 41400045 MEMα ThermoFisher Scientific 12571063 DeadEndTM Fluorometric TUNEL System Promega G3250 Vector Red Alkaline Phosphatase Substrate Kit Vector Laboratories SK-5100 β-Galactosidase Staining Kit Cell Signaling Technology #9860 Vectashield antifade mounting medium Vector H-1000 EZ DNA methylation-lightning kits ZYMO Research D5030 E.Z.N.A.® Total RNA Kit I Omega BIO-TEK R6834 TRIzol® reagent ThermoFisher Scientific 15596018 qScriptTM cDNA SuperMix kit Quantabio 95048 RT2 SYBR® Green qPCR Mastermixes Qiagen 33050 T7 ligase NEB M0318S Bsmb1 NEB R0580 Stbl4 competent cells Invitrogen 11635018 Blasticidin Gibco A1113903 Puromycin Gibco A1113803 Genomic DNA Purification Kit Thermo Scientific K0722 Q5 High-Fidelity DNA Polymerase NEB M0491L Gel Extraction Kit Qiagen 28704 control siNT Dharmacon D-001810-10-05 siNcor2 Dharmacon L-045364-00-0005 jetPRIME transfection reagent Polyplus 101000046 Sodium butyrate Sigma B5887 valproic acid Sigma P4543 protease inhibitors Sigma 04693159001 RIPA buffer ThermoFisher Scientific 89900 Phosphatase inhibitors Sigma 4906845001 BCA Protein Assay Kit Pierce 23250 Gradient polyacrylamide gel Bio-Rad 4561083EDU PVDF membrane Millipore IPVH00010 HRR substrate Millipore WBLUC0500 NEBNext Ultra II Directional RNA Library Prep Kit NEB E7765 Software Epifactors https://epifactors.autosome.org/ GPP sgRNA Design tool https://portals.broadinstitute.org/gpp/public/analysis-tools/ sgrna-design ScreenProcessing Tools https://github.com/mhorlbeck/ScreenProcessing MAGeCK, MAGeCK-VISPR, and MAGeCKFlute Tools https://github.com/WubingZhang/MAGeCKFlute DESeq2 https://bioconductor.org/packages/release/bioc/html/ DESeq2.html SARTools https://github.com/PF2-pasteur-fr/SARTools RRHO2 https://github.com/RRHO2/RRHO2 20 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 17, 2025 from IP 2001:4490:4ead:df7f:1fb:229c:1089:e555.

    Clinical Proteomics:

    Article Title: A primordial germ cell-like-cell platform enables CRISPRi screen for epigenetic fertility modifiers.
    Article Snippet: Reagent/resource Reference or source Identifier or Cat# Snrpn F Bisulfite sequencing TATGTAATATGATATAGTTTAGAAATTAG Snrpn R Bisulfite sequencing AATAAACCCAAATCTAAAATATTTTAATC Snrpn nest F Bisulfite sequencing AATTTGTGTGATGTTTGTAATTATTTGG Snrpn nest R Bisulfite sequencing ATAAAATACACTTTCACTACTAAAATCC H19 F Bisulfite sequencing GAGTATTTAGGAGGTATAAGAATT H19 R Bisulfite sequencing ATCAAAAACTAACATAAACCCCT H19 nest F Bisulfite sequencing GTAAGGAGATTATGTTTATTTTTGG H19 nest R Bisulfite sequencing CCTCATTAATCCCATAACTAT Dnmt3a qF qPCR GACTCGCGTGCAATAACCTTAG Dnmt3a qR qPCR GGTCACTTTCCCTCACTCTGG Dnmt3b qF qPCR CTCGCAAGGTGTGGGCTTTTGTAAC Dnmt3b qR qPCR CTGGGCATCTGTCATCTTTGCACC Ncor2 qF qPCR GATGACCCCATGAAGGTCTACA Ncor2 qR qPCR GGCCAAAGTTCTTAGGGTGCT Gapdh qF qPCR CTTTGTCAAGCTCATTTCCTGG Gapdh qR qPCR TCTTGCTCAGTGTCCTTGC Miseq_Adaptor F MiSeq AATGATACGGCGACCACCGAG ATCTACACACACTCTTTCCCTA CACGACGCTCTTCCGATCTTAT CTTGTGGAAAGGACGAAACACCG Miseq_Adaptor R MiSeq CAAGCAGAAGACGGCATACGAGAT GTGACTGGAGTTCAGACGTGTGCT CTTCCGATCTCTTTAGTTTGTATGT CTGTTGCTATTATGTCTACTATTCTTTCC seqRead Miseq MiSeq GACGCTCTTCCGATCTTATCTTGTGGAA AGGACGAAACACCG CRISPRi F CRISPRi cloning AGGCACTTGCTCGTACGACG CRISPRi R CRISPRi cloning TTAAGGTGCCGGGCCCACAT dCAS9 F dCas9-KRAB expressing CAAGGTGCTGGGCAACACCGA dCAS9 R dCas9-KRAB expressing TCGTCCACCTTGGCCATCTCG Nanog RT F Reverse transcription PCR ACCTGAGCTATAAGCAGGTTAAGAC Prdm1 RT F Reverse transcription PCR gtgacctccatggtggagaag Prdm14 RT F Reverse transcription PCR ggagagaagcccttcaagtgc bGH pA R Reverse transcription PCR GGGGGAGGGGCAAACAACA Tfap2c RT F Reverse transcription PCR GGGGACGGAATGAGATGGC WPRE R Reverse transcription PCR AAGGCATTAAAGCAGCGTATCC rtTAM2 F Reverse transcription PCR ATGTCTAGACTGGACAAGAGC rtTAM2 R Reverse transcription PCR CAGAAAGTCTTGCCATGAC Antibodies DDX4 (Rb) Abcam ab13840 DDX4 (Ms) Abcam ab27591 PLZF (Ms) Active motif 39987 GCNA (Rat) Abcam ab82527 DAZL (Rb) ThermoFisher PA5-116570 NANOS3 (Rb) Abcam ab70001 STRA8 (Rb) Abcam ab49602 SOX9 (Rb) Millipore AB5535 ZO1 (Rb) ThermoFisher 40-2200 18 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 17, 2025 from IP 2001:4490:4ead:df7f:1fb:229c:1089:e555. .. Reagent/resource Reference or source Identifier or Cat# E-Cadherin (Ms) TaKaRa M108 SSEA1 (Ms) Millipore MAB4301 SOX2 (Ms) ThermoFisher MA1-014 H3K9me3 (Rb) Upstate 07-442 H3K27me3 (Rb) EPIGENTEK A-4039-025 Goat anti-Mouse-488 Invitrogen A11001 Goat anti-Rabbit-594 Invitrogen A11012 Goat anti-Rat-594 Invitrogen A11007 Beta-actin (Ms) Sigma 1978 CAS9 (Ms) Abcam ab191468 H3K27ac (Rb) Abcam ab4729 H3 (Rb) Abcam ab1791 Goat anti-Mouse IgG, HRP ThermoFisher 31430 Anti-rabbit IgG, HRP Cell signaling 7074S PE (Phycoerythrin) mouse/rat anti-CD61 Invitrogen 12-0611-82 Alexa Fluor® 647 mouse/human anti-SSEA1 Biolegend 125608 Recombinant DNA pPBhCMV1-Prdm1-pA This study N/A pPBhCMV1-Prdm14-pA This study N/A pPBhCMV1-Nanog-pA Prof. M. Azim Surani N/A PCR4-Shh::lacZ-H11 Addgene #139098 pPyCAG-Pbase Prof. M. Azim Surani N/A pPB-CAG-rtTA-IN Addgene #60612 pSpCas9(BB)-2A-PuroR Addgene #62988 psPAX2 Addgene #12260 pMD2.G Addgene #12259 pLV-tetO-Tfap2c Addgene #70269 pLX_311-KRAB-dCas9 Addgene #96918 pGEM-T Easy vector Promega A1360 pXPR_050 Addgene #96925 Chemicals and other reagents TransIT®-LT1 Transfection Reagent Mirus BioTM MIR2304 GeneticinTM Life Technologies 10131035 Zeocin® InvivoGen ant-zn-05 LIF Peprotech 300-05 PD0325901 Reprocell 04-0006-02 CHIR99021 Reprocell 04-0004-02 poly-L-ornithine Sigma-Aldrich P3655 laminin Corning 354232 bovine plasma fibronectin Sigma-Aldrich F1141 Activin A Peprotech 120-14E bFGF Gibco 13256-029 KSR ThermoFisher 10828010 Dox Sigma D9891 Propidium iodide Invitrogen P3566 © The Author(s) EMBO reports 19 D ow nloaded from https://w w w .em bopress.org on N ovem ber 17, 2025 from IP 2001:4490:4ead:df7f:1fb:229c:1089:e555. .. Reagent/resource Reference or source Identifier or Cat# Goat serum Sigma NS02L GDNF PeproTech 450-44 Insulin-Transferrin-Selenium ThermoFisher Scientific 41400045 MEMα ThermoFisher Scientific 12571063 DeadEndTM Fluorometric TUNEL System Promega G3250 Vector Red Alkaline Phosphatase Substrate Kit Vector Laboratories SK-5100 β-Galactosidase Staining Kit Cell Signaling Technology #9860 Vectashield antifade mounting medium Vector H-1000 EZ DNA methylation-lightning kits ZYMO Research D5030 E.Z.N.A.® Total RNA Kit I Omega BIO-TEK R6834 TRIzol® reagent ThermoFisher Scientific 15596018 qScriptTM cDNA SuperMix kit Quantabio 95048 RT2 SYBR® Green qPCR Mastermixes Qiagen 33050 T7 ligase NEB M0318S Bsmb1 NEB R0580 Stbl4 competent cells Invitrogen 11635018 Blasticidin Gibco A1113903 Puromycin Gibco A1113803 Genomic DNA Purification Kit Thermo Scientific K0722 Q5 High-Fidelity DNA Polymerase NEB M0491L Gel Extraction Kit Qiagen 28704 control siNT Dharmacon D-001810-10-05 siNcor2 Dharmacon L-045364-00-0005 jetPRIME transfection reagent Polyplus 101000046 Sodium butyrate Sigma B5887 valproic acid Sigma P4543 protease inhibitors Sigma 04693159001 RIPA buffer ThermoFisher Scientific 89900 Phosphatase inhibitors Sigma 4906845001 BCA Protein Assay Kit Pierce 23250 Gradient polyacrylamide gel Bio-Rad 4561083EDU PVDF membrane Millipore IPVH00010 HRR substrate Millipore WBLUC0500 NEBNext Ultra II Directional RNA Library Prep Kit NEB E7765 Software Epifactors https://epifactors.autosome.org/ GPP sgRNA Design tool https://portals.broadinstitute.org/gpp/public/analysis-tools/ sgrna-design ScreenProcessing Tools https://github.com/mhorlbeck/ScreenProcessing MAGeCK, MAGeCK-VISPR, and MAGeCKFlute Tools https://github.com/WubingZhang/MAGeCKFlute DESeq2 https://bioconductor.org/packages/release/bioc/html/ DESeq2.html SARTools https://github.com/PF2-pasteur-fr/SARTools RRHO2 https://github.com/RRHO2/RRHO2 20 EMBO reports © The Author(s) D ow nloaded from https://w w w .em bopress.org on N ovem ber 17, 2025 from IP 2001:4490:4ead:df7f:1fb:229c:1089:e555.



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